Calpain-1 Mediated Disorder of Pyrophosphate Metabolism Contributes to Vascular Calcification Induced by oxLDL
Fig 8
Inhibition of ROS in mitochondria reduced calcification of RVSMCs and regulated the expression of ALP.
In one experiment, RVSMCs were treated with or without oxLDL in the presence or absence of calpain-1 siRNA. In the other experiments, RVSMCs were treated with or without oxLDL in the presence or absence of TEMPO. The parameters were measured as described in Materials and Methods. A: Mito-SOX in red color indicating ROS production in mitochondria of RVSMCs (A1: representative photograph; A2: statistical results); B: Calcium deposition in RVSMCs in red and black color (B1: representative photograph; B2: statistical results); C: PPi content in medium; D: ALP activity in medium; E: ALP activity in RVSMCs; F: ALP mRNA expression in RVSMCs; G: ALP protein expression in RVSMCs (upper: representative photograph; lower: statistical results). Results were expressed as mean±S.D. n = 4. *: P<0.05 was considered statistically significant.