Analysis of the siRNA-Mediated Gene Silencing Process Targeting Three Homologous Genes Controlling Soybean Seed Oil Quality
Fig 8
5’RACE on GmFAD3A and GmFAD3C mRNAs in T5 RNAi lines.
Arrows indicate the inferred cleavage sites and numbers above represent the fractions of cloned 5’ RACE PCR products terminating at this position. Degradation sites detected with high frequency are highlighted in red, and those present across the three RNAi lines are highlighted with asterisks. (A) Summary of the 5’ RACE analysis performed on the 318-bp region of GmFAD3A mRNA. (B) and (C) Summary of the 5’ RACE analysis performed on the corresponding region of GmFAD3C mRNA.