In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues
Fig 2
Populations of 2W:I-Ab tetramer+ cells detected in situ in NALT co-stained with CD4 antibodies.
(A) Representative image of a NALT section stained with 2W:I-Ab tetramers with no TSA amplification (red) and CD4 antibodies (blue). 2W:I-Ab tetramer+ cells that were co-labeled with CD4 are indicated by arrowheads. In this field, all of the tetramer+ cells are CD4+. For this image, several confocal z-scan fields were collected using a 20X objective and reconstructed as a montage. (B) Shows two 2W:I-Ab tetramer-binding CD4+ T cells (indicated by arrowheads). (C) Shows a 2W:I-Ab tetramer-binding cell (red) from another field that is not co-stained with CD4 antibodies. (B) and (C) are confocal z-scans collected using the same parameters with a 60X objective.