Transcriptome Characterization of Cymbidium sinense 'Dharma' Using 454 Pyrosequencing and Its Application in the Identification of Genes Associated with Leaf Color Variation
Fig 10
The qRT-PCR analysis of the expressions of two enzyme-encoding genes in chl degradation pathway (A) and five key enzyme-encoding genes in chl biosynthesis pathway (B). The y-axis indicates fold change in expression among the samples. The Lg(Relative Quantitation) of the genes in the wild-type leaves was calibrated as zero. RNA was extracted from six-month-old normal growth leaves. ACT gene served as the internal control. Error bars indicate the standard deviation of the mean (SD) (n = 3). Three biological replicates were analyzed, with similar results.