Mitochondrial Reshaping Accompanies Neural Differentiation in the Developing Spinal Cord
Fig 6
Differential expression of effectors of mitochondrial dynamics in E1.5 and E3.5 chicken neural tube extracts.
Representative immunoblot showing the expression of the mitochondrial dynamics proteins, DRP1, MFN2 and OPA1, as well as mitochondrial respiratory complexes V, III and II and HSP60 protein in E1.5 and E3.5 chicken neural tubes extracts, with actin used as control. While DRP1 and MFN2 are represented as single bands of similar intensities at both ages, staining of the OPA1 isoforms reveals an increase in the longest isoform in the E3.5 extract compared with the E1.5 extract.