Immunoproteasome Deficiency Protects in the Retina after Optic Nerve Crush
Fig 2
Optic Nerve Crush Increases Stress and Correlates with an Increase in Immunoproteasome Subunits in WT Retina.
(A and B) Protein was isolated from WT (●), L2 (□), and L7M1 (Δ) mouse retinas at the indicated time points after ONC. Results are the optical density of immunoproteasome subunits (A) LMP7 (β5i) and (B) LMP2 (β1i), and are the mean ± S.E. of 3–22** mice/group compared to control (day 0). (*, p≤0.05 (WT), and #, p≤0.01 (L2) by one-way ANOVA with Dunnett’s post-test compared to the ‘day 0’ values). (** All 36 day WT mouse data represents 2 mice/group). (C) WT retina tissue sections stained with anti-GFAP in WT retina before and after optic nerve crush (11 days). Image was taken with a 40x objective. (D) Protein was isolated from WT (●), L2 (■), and L7M1 (▲) retinas at the indicated time points after ONC. Results are the optical density of the GFAP protein content, and are the mean ± S.E. of 3–15 mice/group compared to control (day 0) for each mouse strain. (*, p≤0.001 (WT), #, p≤0.01 (L2) by one-way ANOVA with Dunnett’s post-test compared to the ‘Day 0’ values). (E) GFAP and LMP7 protein content isolated from WT (●) or L2 (□) mice are shown as a correlation. (R2 = 0.2292; p≤0.01 (WT), R2 = 0.1620; p≤0.01 (L2), by Pearson Correlation). (F) GFAP and LMP2 protein content from WT (●) or (Δ) L7M1 mice are shown as a correlation. (R2 = 0.2860; p≤0.001 (WT), R2 = 0.0126, p = 0.57 (L7M1), by Pearson Correlation).