Promotion of Viral IRES-Mediated Translation Initiation under Mild Hypothermia
Fig 5
Temperature sensitivity of cellular IRES-mediated translation.
NIH3T3 cells were transfected with a HRV, Rbm3, Apaf-1, c-myc, BIP, CAT-1 construct or a control pRF reporter construct and 24 hours later were incubated at 37°C (white bar) or 30°C (black bar) for 8 hours. Firefly and Renilla luciferase units were measured using the Dual-Luciferase Reporter Assay System. Firefly/Renilla represents the ratio of viral IRES-mediated translation to cap-dependent translation. Data are expressed as the mean ± SE of 3 independent samples. Statistical analysis was conducted using two-way ANOVA and multiple comparison test. *p<0.01.