Extremely Sparse Olfactory Inputs Are Sufficient to Mediate Innate Aversion in Drosophila
Fig 3
Aversion after ORN-specific restoration of synaptic transmission.
(A-H) Pebbled-GAL4 expression in specific glomeruli is suppressed by Ir64a-GAL80 (A, B), Or22a-GAL80 (C, D), Or85a-GAL80 (E, F), and Or42b-GAL80 (G, H). White texts label the glomeruli predicted from published work; yellow texts label the additionally suppressed glomeruli. Glomeruli with reduced expression of ORN outputs are outlined. Each image is representative of 5 brains of the same genotype. (I) A representative scheme of using Or22a-GAL80 to restore inputs to DM2 glomerulus in the pan-ORN inactivation background, same legend as Fig 2A. (J, K) Aversion in Pebbled AND ey > shits1 flies to E2-hexenal (J, n ≥ 3) and acetic acid (K, n ≥ 3) is recued by GAL80s, and Or42b-GAL80 causes less aversion to E2-hexenal than Or22a-GAL80 (J). The stars right below each bar indicate statistical significance as compared to the non-GAL80 control.