Incorporation of Porcine Adenovirus 4 Fiber Protein Enhances Infectivity of Adenovirus Vector on Dendritic Cells: Implications for Immune-Mediated Cancer Therapy
Fig 2
A. Ad5PK enhances DC gene transfer compared to previously reported Ad5 constructs. Human immature DCs were infected with Ad5Luc1, Ad5Luc.FF/CD40L, Ad5/3Luc1 or Ad5Luc1-PK at an MOI of 300 VP/cell. Luciferase activity (relative light unit, RLU) was determined in 48hr post infection. All fiber modified Ad5 vectors showed infectivity enhancement in iDC. *, +, and # indicate p = 0.02, p = 0.0005 and p<0.0001, respectively. B. Murine iDC were plated in triplicate and infected with Ad5Luc1-PK and Ad5Luc1 (control) at an MOI of 300 VP/cell. Luciferase activities were measured after 48 hrs. Ad5Luc1-PK showed a 4-fold increase in murine iDCs of gene transfer activity (“#” indicates p<.003).