STIM1 Is a Novel Component of ER-Chlamydia trachomatis Inclusion Membrane Contact Sites
Fig 8
STIM1 depletion does not affect C. trachomatis growth.
HeLa cells were transfected with control siRNA (GFPsi) or a pool of CERT siRNA (CERTsi) or a pool of siRNA against STIM1 (STIM1si) for 3 days and infected with a strain of C. trachomatis expressing mCherry. (A) For each condition, the surface area of the inclusions was determined (Arbitrary Units). Each circle, square or triangle represents data from a single inclusion. (B) The number of infectious bacteria (IFUs/ml) was determined 48h p.i. (A-B) Data show the mean and standard deviation of biological triplicate of a representative experiment. ***P<0.0001 (student’s t test).