REGULATOR OF BULB BIOGENESIS1 (RBB1) Is Involved in Vacuole Bulb Formation in Arabidopsis
Fig 1
regulator of bulb biogenesis1 contains many bulbs in many cell types.
(a-h) rbb1-1 mutants have more bulbs than the parental line. GFP-TIP2;1 localization in the parental line (WT, a-d) or rbb1-1 (e-h). Four-day-old seedlings were imaged by confocal microscopy to visualize morphology of the vacuole in the epidermis of hypocotyl (a, e) and cotyledon (b, f), and in root epidermis and cortex (c, g). The bulb phenotype was also observed in rosette leaves of 6-week-old plants (d, h). Scale bar = 20 μm. (i-n) GFP-TIP2;1 and RFP-TIP1;1 co-localize in rbb1-1 bulbs. The hypocotyls of 4-day-old dark-grown seedlings expressing GFP-TIP2;1 (green, i, l) and RFP-TIP1;1 (magenta, j, m) in the parental line (i-k) and rbb1-1 mutants (l-n) are shown. Merged images (k, n) are also shown. Scale bar: 20 μm. (o) rbb1-1 mutants do not accumulate higher levels of GFP-TIP2;1 in seedlings. Immunoblot of GFP-TIP2;1 accumulation in the parental line and rbb1-1 using antibodies against GFP (α-GFP) and Calreticulin (α-CRT) as loading control.