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A High Throughput Protein Microarray Approach to Classify HIV Monoclonal Antibodies and Variant Antigens

Fig 3

Cluster analysis. Arrays were probed with mAbs diluted in a series of 8 half log concentrations as described in the text.

The heat map displays intensity with orange (>1.5–3.0 x 104) and red (>3.0 x 104) showing the strongest, green (<5.0 x 103) the weakest, and yellow (>0.5–1.5 x 104) intermediate according to Fig 1. MeV v4.6 (TM4Microarray Software Suite, www.tm4.org) was used to perform the hierarchical clustering analysis using average linkage clustering. Samples are clustered according to response against protein antigen spots (1.0 ng per spot) on the array.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0125581.g003