The MicroRNA-217 Functions as a Potential Tumor Suppressor in Gastric Cancer by Targeting GPC5
Fig 3
miR-217 inhibits GC cell proliferation and invasion.
(A) Real-time RT-PCR analysis of miR-217 in HGC-27 cells upon transfection ofmiR-217 mimic. The expression of miR-217 in HGC-27 cells transfected with miR-217 mimics was up-regulated.U6 snRNA was used as internal control. (B) The expression of miR-217 in HGC-27 cells transfected with miR-217inhibitor was down-regulated.U6 snRNA was used as internal control. (C) Ectopic miR‑217 expression significantly inhibited cell proliferation, as demonstrated by CCK8 assay. (D) Inhibition of miR-217 expression significantly promoted cell proliferation, as demonstrated by CCK8 assay. (E) Invasion analysis of HGC-27cells after treatment withmiR-217 mimics, inhibitors or scramble or control; the relative ratio of invasive cells per field is shown below, *p<0.05, ** p<0.01, and ***p<0.001.