Hepatitis C Virus NS3 Mediated Microglial Inflammation via TLR2/TLR6 MyD88/NF-κB Pathway and Toll Like Receptor Ligand Treatment Furnished Immune Tolerance
Fig 4
TLR6 was involved in NS3 mediated microglial activation.
Microglia was exposed to 20 ng/ml of NS3 for different time points and cells were stained for TLR6 protein expression. (A-B) Flow cytometry results indicates that TLR6 expression was significantly high from 1 hr to 3 hr time point. (C) Also NS3 exposed microglia stained brighter for TLR6 at 3 hr time point compared to the control cells. (D-F) TLR6 expression was silenced by TLR6 specific siRNA and the (G) TLR6 silenced cells secreted significantly less IL-6. Data is expressed as mean (n = 3) ± SE. * p < 0.05. Scale bar corresponds to 50 micron.