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The Bactofilin Cytoskeleton Protein BacM of Myxococcus xanthus Forms an Extended β-Sheet Structure Likely Mediated by Hydrophobic Interactions

Fig 3

Structure of BacM fibers, ribbons, and filaments after dialysis against various buffers.

Exogenously expressed and purified BacM polymerizes into different structures upon dialysis against different buffers. (A) In a phosphate-citric acid buffer (pH 6.0, top), or at high concentrations (100 μM, bottom), large ribbon-like structures were observed. (B) In Tris, CHES or CAPS buffers (pH 7.5, 9.5 or 10.5, respectively), 10 nm fibers resembling those isolated from M. xanthus cells predominate (top row). The addition of 20 mM glycine to the buffers (bottom row) favors the formation of 3 nm filaments at increasing abundance with increasing pH. Scale bar = 50 nm.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0121074.g003