Single Cell Transcriptome Amplification with MALBAC
Fig 4
Gene expression heat map of EMT-related genes.
Genes related to epithelial-mesenchymal transition (EMT) are differentially expressed across the three germ layers. Among them, FGF10 and Snai1 are significantly overexpressed in the mesoderm, whereas E-cadherin and Sox3 are depleted. At the same time, Eomes and Mesp1 are highly expressed in the mesoderm, although Mesp2 is not significantly expressed. Other EMT-related genes, including CDH2, Wnt5a, Wnt3, Hmga2, Smad1, and Fgf10, are also enriched in the mesoderm, which confirms the cellular transitions during gastrulation.