Multiple RNA Processing Defects and Impaired Chloroplast Function in Plants Deficient in the Organellar Protein-Only RNase P Enzyme
Fig 8
Investigation of the relationship between tRNA editing and 5’ end maturation.
(A) Analysis of the editing status of tRNA-Arg(ACG) in the chloroplast. The A-to-I editing event changes the adenosine in the wobble position (position 34) of the ACG anticodon to inosine, which is read as guanosine (boxed) by reverse transcriptases [5,6]. Editing efficiencies are shown for the wild type (WT) and the most affected RNAi line (RNAi-2). (B) Assessment of the efficiency of tRNA-Arg(ACG) processing by RNase P in the absence of A-to-I editing. tada-1 is a knock-out allele of the specific adenosine deaminase that edits the anticodon of the plastid tRNA-Arg(ACG) [5,6]. Due to the weak exposure of the blot, the mature tRNA in the RNAi line is hardly visible.