TORC1 Promotes Phosphorylation of Ribosomal Protein S6 via the AGC Kinase Ypk3 in Saccharomyces cerevisiae
Fig 4
Phenotypic characterization of the Rps6 phosphorylation deficiency.
A) WT strain BY4742 and the indicated mutants were tested for growth in YPD medium. Growth was monitored at the indicated times. Data are presented as the mean ± S.E.M. of triplicate determinations and correspond to a representative experiment among three. Statistical analysis was performed by using two-way ANOVA. WT vs. rp6bΔ (significant: p < 0.001), WT vs. rps6aS232A S233A rp6bΔ (significant: p = 0.001), rp6bΔ vs. rps6aS232A S233A rp6bΔ (not significant: p = 0.153). B) Cell size of the indicated strains was measured. Two-way ANOVA analysis was performed. **, p<0.01; ns, not significant. C) WT strain BY4742 and the indicated mutants were spotted onto YPD plates containing either 4 ng/ml of rapamycin or drug vehicle alone. Plates were grown for 3 days at 30°C. Pictures correspond to a representative experiment among three. D) Model of regulation of Rps6 phosphorylation by TORC1-Ypk3.