Curcumin and Emodin Down-Regulate TGF-β Signaling Pathway in Human Cervical Cancer Cells
Fig 4
Effects of curcumin, emodin and TGF-β on cell invasion.
SiHa (25,000) and HeLa (50,000) cells seeded onto Boyden chamber coated with matrigel, were treated with curcumin, emodin and/or TGF-β for 48h. Post treatment, cells were stained as described in Materials and Methods, and imaged using microscope (A). Quantification of invaded cells (B and C) was done by reading the absorbance of crystal violet at 595nm. Percentage invasion was calculated as fold change with respect to untreated control (n = 3, mean ± S.E.M.)