Engineering More Stable, Selectable Marker-Free Autoluminescent Mycobacteria by One Step
Fig 1
The plasmids constructed in this study for transforming into mycobacteria to create unmarked autoluminescent mycobacteria.
oriE, origin region of E. coli; Hsp60, the strong mycobacterial promoter; luxCDABE, the operon for producing autoluminescence; bla, ampicillin resistance gene; Kan, KAN resistance gene; res, the transposonγδ resolvase action site; attP, mycobacteriophage L5 attachment site; int, integrase gene; int’, the remaining part of integrase gene; attB, attachment site from the mycobacterial genome corresponding to attP; oriM, origin region of mycobacteria; Hyg, HYG resistance gene; dif, the recombinases XerCD action site.