Interferon-γ Suppresses Intestinal Epithelial Aquaporin-1 Expression via Janus Kinase and STAT3 Activation
Fig 4
The STAT1/IRF-1 pathway is not required for the IFNγ-induced reduction in AQP1 expression.
(A) Immunoblots showing STAT1, IRF-1, AQP1 and actin expression in CMT93 cells. Cells were pretreated with STAT1 or scrambled siRNA (80 nM for 24 hr) or with the transfection control medium (Lipofectamine Control), followed by either serum-free medium or IFNγ (10 ng/mL for 24 hr). (B) Densitometry graph of total (t) STAT1 normalized to actin expression. (C) Densitometry graph of AQP1 normalized to actin expression. *p < 0.05, **p < 0.01; blots are representative of 3 separate experiments.