The Roles of Integrins in Function of Human Neutrophils after Their Migration through Endothelium into Interstitial Matrix
Fig 7
Effects of inhibition of integrin function on adhesion to TNF-treated EC and migration into subendothelial matrix.
HUVEC were stimulated with 100U/ml TNF for 4 h and neutrophils were added and allowed to adhere for 10 min before non-adherent cells were washed off and data collected at 15 min, 1 h, 3 h and 24 h. A,C: Total adherent neutrophils after 15min (% of those added). B,D: Average depth penetrated by the neutrophils that had transmigrated. In A, B, neutrophils were untreated, or pre-treated with antibody against β1- or against β3-integrin. In C, D, neutrophils were untreated, or pre-treated with antibodies against β1-, β2- and β3-integrin, or with a combination of non-specific IgG with matching isotypes. In C, ANOVA showed a significant effect of treatment (p<0.01 in each case); ** = p<0.01 by Dunnett test compared to untreated. In B, D, ANOVA showed significant effect of time but not treatment (p<0.01 in each case); individually, no treatment showed a significant effect on depth of penetration even for the four combined time points. Data are mean ± SEM from 4 experiments (A,B) or three experiments (C,D).