Programmed Evolution for Optimization of Orthogonal Metabolic Output in Bacteria
Fig 6
Starting Population for Programmed Evolution.
(A) An ampicillin resistance plasmid carries variation in the strength of promoters and RBS elements as well as the low and high copy number origins of replication. (B) A chloramphenicol resistance plasmid carries chaperones DNA KJE, Trigger Factor, and Gro ESL chaperones individually and in two combinations (see Methods for details).