Transoceanic Spreading of Pathogenic Strains of Vibrio parahaemolyticus with Distinctive Genetic Signatures in the recA Gene
Fig 4
NeighborNet graph generated using the BIGSdb Genome Comparator tool implemented within the PubMLST website [34,35] with 2005 variable loci identified after using as a reference (ref) RIMD2210633 chromosome I (3079 genes) of available V. parahaemolyticus genomes at Genbank (Table A in S1 File for the list of the strains) and strain 090–96 (Peru 1996), showing that this strain is closed related to BB22OP (ST88) and the two other ST189 strains and far related to other strains containing fragmented recA genes with similar insertion (S130 and S134, both ST527).
This clearly suggests acquisition of this region by HGT. A similar graph was obtained with chromosome II of the same strain as reference (data not shown). Distances between taxa are calculated as the number of loci with different allele sequences.