Identification of Aortic Arch-Specific Quantitative Trait Loci for Atherosclerosis by an Intercross of DBA/2J and 129S6 Apolipoprotein E-Deficient Mice
Fig 5
DBA-allele dependent upregulation of Stab2 and its ligand hyaluronan.
(A) Plasma hyaluronan concentrations in the wild-type male C57BL/6, 129S6 and DBA2/J mice. Box-and-whisker plots: midline, median; box, 25th and 75th percentiles; whiskers, 1.5× interquartile range; dots, outliers. Numbers of mice were indicated below the plots. (B) Plasma hyaluronan concentrations in a subset of F2 males and females of 129-apoE × DBA-apoE. Mice were grouped by the genotype of rs13480630 on Chr 10, which is located near Stab2. Differences were compared by Kruskal–Wallis test followed by Steel-Dwass test for (A) and (B). (C) Effects of other hyaluronan metabolic loci on plasma hyaluronan concentrations. The same set of F2 population was grouped into 129-homo, Hetero and DBA-homo according to the genotype of rs13459148 on Chr 17 (near Has1), rs13482628 on Chr 15 (near Has2), rs3667255 on Chr 8 (near Has3), rs13480325 on Chr 9 (near Hayl1–3), rs13476631 on Chr 2 (near Cd44), rs13479497 on Chr 7 (near Lyve1) and rs13480973 on Chr 11 (near Hmmr). Kruskal–Wallis test was used for the multiple comparison. (D) Expression levels of Stab2 in various tissues from B6 (black filled bars), 129 (gray filled bars) and DBA (open bars). Data are relative to those in the B6 liver (= 100) assessed by quantitative RT-PCR. **P < 0.01, ***P < 0.001 vs. B6; ##P < 0.01, ###P < 0.001 vs. 129 (one-way ANOVA followed by Tukey-Kramer’s HSD test). Data were shown as the mean ± SD. Sample numbers were indicated in the bars.