Catalytic Efficiency of Chitinase-D on Insoluble Chitinous Substrates Was Improved by Fusing Auxiliary Domains
Figure 4
Degradation of α- or β-chitin by SpChiD fusion chimeras.
(A) α-chitin (or) (B) β-chitin (2.5% (w/v)) was incubated with 1μM each of SpChiD or its fusion chimeras at 40°C for 1 h, in 50 mM sodium acetate buffer, pH 5.2. The values are based on mean ±SD of three identical experiments. Data were analyzed by one-way ANOVA followed by Tukey's Multiple Comparison Test. Statistical significance was determined at p≤0.05.