Sodium Butyrate Promotes the Differentiation of Rat Bone Marrow Mesenchymal Stem Cells to Smooth Muscle Cells through Histone Acetylation
Figure 2
Immunofluorescence analysis of MSC specific protein expression in NaB-treated MSCs.
MSCs were treated with 1 mmol/L NaB for 48 h, stained with FITC-conjugated anti-α-SMA, calponin or SM-MHC antibodies, and observed under a fluorescence microscope. The untreated MSCs were used as negative control and the primary SMCs were used as positive control. The isotype antibody was used as a background control. DAPI was used to stain the nuclei. Scale bar = 25 µm.