Acute Graft-Versus-Host Disease of the Kidney in Allogeneic Rat Bone Marrow Transplantation
Figure 8
Infiltrating cells in the kidney in acute GVHD after allogeneic bone marrow transplantation (BMT).
Double immunofluorescence stain by fluorescence antibody technique against CD3+ (A) and CD8+ (B), and their merged image (C) indicated that, in the kidney with acute GVHD on day 28, CD8+ T-cells (arrow in A–C) infiltrated the kidney. In addition, CD4+ T-cells (arrow in D–F) were also noted in inflammation, indicating that not only class I-restricted T cell-mediated reactions but also class II-restricted T cell-mediated reactions developed in renal acute GVHD. Double immunofluorescence stain against RT1Aa,b (G) and CD45 (H), and their merged image (I) indicated that, in the kidney with acute GVHD on day 28, almost all CD45+ leukocytes (arrow in G–I) were expressed rat RT1Aa, b, suggesting the infiltration of donor-type leukocytes in acute renal GVHD.