Intrinsically Disordered and Pliable Starmaker-Like Protein from Medaka (Oryzias latipes) Controls the Formation of Calcium Carbonate Crystals
Figure 8
Analysis of the impact of Stm-l on the calcium carbonate formation process.
(A) The number of calcium carbonate crystals was determined by counting them using 3 different SEM 1.2×0.9 mm images for each experimental conditions and then calculating the average number of crystals. (B) The average size of the calcium carbonate crystals was determined from SEM images by measuring crystals for their edge length or diameter, for prismatic and rounded crystals, respectively. Panels (A) and (B) show respective data obtained in the absence of any protein, in the presence of trypsin at a concentration of 100 µg/mL, and in the presence of Stm-l at a concentration of 50 µg/mL. Calcium ion concentrations were 5 mM, 10 mM, and 20 mM (black, white, and checked bars respectively).