Domain Dissection of AvrRxo1 for Suppressor, Avirulence and Cytotoxicity Functions
Figure 6
Function analysis of the four AvrRxo1 mutants expresssed in PXO99A.
(A) The phenotype of interactions between maize lines B73 and four AvrRxo1 mutants: AvrRxo1(H71A), AvrRxo1(G165A), AvrRxo1(K166N), and AvrRxo1(nls). Mutants AvrRxo1(H71A) and AvrRxo1(G165A) induce HR at 2 dpi, however mutants AvrRxo1(K166N) and AvrRxo1(nls) are abolsihed to elicit HR on B73. (B) The phenotype of suppression of non-host HR in N. benthamiana caused by PXO99A. PXO99A(pHM1) was used as negative control and PXO99A(pHM:avrRxo1) as positive control. PXO99A containing AvrRxo1(H71A) (6) did not elicit the non-host HR in N. benthamiana, while PXO99A containing the fragments AvrRxo1(G165A) (3), AvrRxo1(K166N) (4), and nls (5) can still elicit non-host HR. RT-PCR was used to confirm the expression of avrRxo1 in inoculated leaves. All experiments were repeated three times with similar results.