Sleeping Beauty Mouse Models Identify Candidate Genes Involved in Gliomagenesis
Figure 1
GFAP-SB11 transgenics express functional transposase in a subset of GFAP+ cells.
Two lines (A and B) were established and used for these experiments. SB = SB transposase, T2 = T2/onc, LV = lateral ventricle. A) Immunofluorescence for GFAP (red) and SB (green). Nuclei are stained with DAPI. Arrows indicate examples of SB+ GFAP+ cells while asterisks indicate examples of SB− GFAP+ cells. Scale bars are 20 µm. B) PCR based excision assay showing that transposons have mobilized in the brains of SB+T2+ but not SB−T2+ or SB+T2− mice from each line. A control PCR demonstrates that genomic DNA is present for all samples.