Niacin Activates the PI3K/Akt Cascade via PKC- and EGFR-Transactivation-Dependent Pathways through Hydroxyl-Carboxylic Acid Receptor 2
Figure 6
The Gβγ subunit involved in HCA2 signaling mediates Akt phosphorylation.
CHO-HCA2 cells (A) were transfected with β-ARK1-CT for 48 h and CHO-K1 cells (B) were co-transfected with Gα-transducin and either pCDNA3.1 or HCA2 for 48 h, followed by niacin stimulation and Akt (Ser473) phosphorylation detection. The data shown are representative of at least three independent experiments. The data were analyzed using Student’s t test (*, p<0.05; **, p<0.01; ***, p<0.001).