miR-18a Inhibits CDC42 and Plays a Tumour Suppressor Role in Colorectal Cancer Cells
Figure 5
CDC42 expression is reduced by miR-18a but increased by miR-19a and b in HCT116 cells.
(A) CDC42 mRNA levels in NC, miR-18a mimic, and miR-18a, 19a and b mimic transfected cells at 48 h post-transfection (B) CDC42 mRNA levels in NC and miR-18a inhibitor transfected cells at 48 h post-transfection. The mean ± SEM of 6 cell culture replicates is shown for each and expression is normalised to ACTB. * P<0.05. (C and D) Western blot of CDC42 protein levels in NC, miR-18a mimic, and miR-18a, 19a and b mimic transfected cells at 48 h post-transfection. Densitometry graph shows the mean ± SEM of 3 cell culture replicates for each and expression is normalised to GAPDH * P<0.05.