Development of an OP9 Derived Cell Line as a Robust Model to Rapidly Study Adipocyte Differentiation
Figure 4
Automated lipid accumulation detection enables high throughput adipogenesis assay.
Analysis starts with the raw image as captured by microscopy system. The program draws a boundary around the DAPI foci, identifying the nuclei (shown in blue). A cell outline is drawn around each nucleus, identifying the cell boundary. Lastly, the intensity of Nile Red staining within the cell boundary is quantified (Nile Red shown in red). The analysis steps are shown on representative images of differentiating OP9 clone K cells over the course of 72 hours.