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Hypoxia-Controlled EphA3 Marks a Human Endometrium-Derived Multipotent Mesenchymal Stromal Cell that Supports Vascular Growth

Figure 3

Phenotypic characterization of EphA3+ endometrial stromal cells.

(A) Immunofluorescence analysis of EphA3+eSCs using antibodies to EphA3 (red) and indicated antigens (green), characteristic of an MSC cell surface expression profile. Individual fluorescent channels and merged images are shown, 25 µm scale bars. (B) Cell surface expression profile of indicated antigens on EphA3+eSCs was assessed by flow cytometry; filled peaks represent isotype-matched controls. (C) The mRNA expression levels of indicated genes (relative to β-actin) were assessed in EphA3+eSCs by qRT-PCR. Mean and standard deviation (n = 3) are shown.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0112106.g003