Decreased MicroRNA-125a-3p Contributes to Upregulation of p38 MAPK in Rat Trigeminal Ganglions with Orofacial Inflammatory Pain
Figure 4
Effects of miR-125a-3p on p38 MAPK expression and luciferase activity in ND8/34 cells.
(A) Binding sites alignment of the mature miR-125a-3p and the 3′UTR of rat p38 MAPK gene (left) and base pair difference between wild type (WT) and mutant type (MT) of p38 PAPK gene 3′ UTR (right). (B) The expression of p38 MAPK mRNA due to the transfection of 0, 25 or 50 nM miR-125a-3p mimic, inhibitor or non-target control (NT-C) in ND8/34 cells detected by qPCR. (C) The luciferase activity of p38 MAPK 3′UTR WT or 3′UTR MT due to the transfection of 50 nM miR-125a-3p mimic, inhibitor or NT-C in ND8/34 cells. * p<0.05, and ** p<0.01.