Screening the Expression of ABCB6 in Erythrocytes Reveals an Unexpectedly High Frequency of Lan Mutations in Healthy Individuals
Figure 4
Determination of the subcellular localization of ABCB6 variants.
A. Double immunofluorescence labeling and laser-scanning confocal microscopy images of HeLa cells overexpressing ABCB6-HA, ABCB6-HA-G588S, ABCB6-HA-R192Q or ABCB6-HA-R192W, probed with the ABCB6-567 antibody (green) [9] in the context of LysoTracker (red) or Calnexin (red). B. Colocalization of ABCB6-variants with various intracellular markers. Bars represesent averaged Pearson’s coefficients +/− standard deviation for each subcellular marker from 5 random pictures. Blue: ABCB6-HA; red: ABCB6-HA-G588S; green: ABCB6-HA-R192Q; purple: ABCB6-HA-R192W.