The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects
Figure 6
Gene expression and protein levels in vivo.
Collagen I (A) and collagen II (B) gene expression levels over 3 months were determined using qRT-PCR, and western blotting was used to evaluate the collagen II, aggrecan, sox9 and collagen I protein levels in the regeneration area (C). The scaffold notably stimulated the expression of the cartilage-related markers, but not collagen I. ♦ Statistically significant difference compared with the control group (p<0.05); • Statistically significant difference compared with the control group (p<0.01); ▪ Statistically significant difference compared with the blank group (p<0.05).