TRAIL-Mediated Apoptosis in Breast Cancer Cells Cultured as 3D Spheroids
Figure 6
COX-2 inhibitor treatment upregulates the expression of DR4 and DR5 in BT20 and MCF7 spheroids.
(A) Flow cytometry histograms showing the effect of 100 µM NS-398 treatment on death receptor expression in BT20 and MCF7 cells cultured as spheroids. (B) Western blot analysis of whole cell lysates from untreated and NS-398 treated BT20 and MCF7 spheroids for DR4 and DR5 expression. (C) Flow cytometry scatter plots showing the effect of NS-398 treatment on the prevalence of CD44hiCD24lo population in BT20 and MCF7 cells cultured as spheroids.