MiR-30a-3p Negatively Regulates BAFF Synthesis in Systemic Sclerosis and Rheumatoid Arthritis Fibroblasts
Figure 6
MiR-30a-3p represses BAFF secretion by healthy FLS and HDF.
A, B. miR-30a-3p expression was determined by RT-qPCR in NFLS (n = 4)/RAFLS (n = 4) (A) and NHDF (n = 3)/SScHDF (n = 4) (B) stimulated with Poly(I:C) (10 µg/mL), IFN-γ (0.1 or 5 ng/mL) or medium for 48 h and 72 h. Results were normalized to U6snRNA and expressed as fold change compared with samples from RAFLS (A) or SScHDF (B) incubated with medium. C. NFLS (n = 3) and NHDF (n = 3) were transfected with miR-30-3p antisense oligonucleotides (20 pM/sample) or with an AllStars negative control (CT). After 24 h, cells were activated with Poly(I:C) (10 µg/mL), IFN-γ (0.1 or 5 ng/mL) or medium for 72 h. BAFF release was determined by ELISA in culture supernatants. D. NHDF (n = 3) transfected with miR-30-3p antisense, were stimulated with poly (I:C). The supernatant was then treated with control IgG or with anti-BAFF antibodies and added to purified B cells. B cells survival was next evaluated as in panel Figure 5. *p<0.05; **p<0.01.