LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2
Figure 5
Overexpression of LOC401317 inhibits HNE2 cell growth in xenografted tumors.
Untreated HNE2 cells (mock) as well as HNE2 cells that were stably transfected with empty vector (vector) or an LOC401317 overexpression plasmid (LOC401317) were injected subcutaneously into the right flanks of nude mice. (A) Xenograft tumor sizes were monitored by measuring every 5 days, between days 10–35 following injection (n = 10 mice per group). Error bars represent SEM, **P<0.01. (B) Mice were sacrificed at 35 days post-injection. (C) Xenografted tumors were separated and their sizes were measured. Formalin-fixed, paraffin-embedded tissues were prepared from xenografted tumors for subsequent in situ hybridization (ISH) or immunohistochemistry (IHC) staining.