TNFα-Mediated Loss of β-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells
Figure 5
The CM treatment increases cytoplasmic β-catenin accumulation correlating with loss of nuclear NKX3.1 expression.
Tissue sections (containing normal, PIA, H-PIN and PCa regions n = 42, 38, 24 and 24 respectively) were cut from 14 radical prostatectomy specimens and analyzed. The adjacent sections were stained with hematoxylin-eosin dye (A–D), β-catenin (E–H) and NKX3.1 (I–L) antibody to correlate the expression changes in in vivo samples. While glands from the normal prostate exhibited nuclear staining for β-catenin similar to PCa, the atrophy, H-PIN and PCa regions demonstrated remarkable increases in cytoplasmic staining. The representative images were taken from normal glands (A, E, I) the atropy (B, F, J), H-PIN lesions (C, G, K) and prostate adenocarcinoma (D, H, L) samples. The relative intensity from analyzed sections and statistical significance values were also given in comparison to normal sections (M). Histogram plot shows the variation of β-catenin expression between stages (N). The images were taken with a 20× objective. Also, brown colored arrows show the atrophy glands.