Eosinophils and Megakaryocytes Support the Early Growth of Murine MOPC315 Myeloma Cells in Their Bone Marrow Niches
Figure 5
MOPC315.BM myeloma cell growth is supported by co-cultured megakaryocytes.
MOPC315.BM cells were cultured at low densities (1000 cells/well) in 96 well plates alone (MM) or together with in vitro-generated megakaryocytes (MK), CD4+ T cells (TC) or IgD+ B cells (BC) in medium supplemented with either 1% or 10% FCS, as indicated. Ratio of myeloma cells to feeder cells was 1ā¶10. After two days of culture the numbers of viable (AnnexinV-, DAPI-) MOPC315.BM cells (GFP+, CD138+) were quantified by flow cytometry. Each dot represents myeloma cell counts from a single well. Data are representative for four independent experiments. Mean +/ā SEM are shown (statistics: Mann-Whitney U test; * = Pā¤0.05).