Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Eosinophils and Megakaryocytes Support the Early Growth of Murine MOPC315 Myeloma Cells in Their Bone Marrow Niches

Figure 5

MOPC315.BM myeloma cell growth is supported by co-cultured megakaryocytes.

MOPC315.BM cells were cultured at low densities (1000 cells/well) in 96 well plates alone (MM) or together with in vitro-generated megakaryocytes (MK), CD4+ T cells (TC) or IgD+ B cells (BC) in medium supplemented with either 1% or 10% FCS, as indicated. Ratio of myeloma cells to feeder cells was 1āˆ¶10. After two days of culture the numbers of viable (AnnexinV-, DAPI-) MOPC315.BM cells (GFP+, CD138+) were quantified by flow cytometry. Each dot represents myeloma cell counts from a single well. Data are representative for four independent experiments. Mean +/āˆ’ SEM are shown (statistics: Mann-Whitney U test; * = Pā‰¤0.05).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0109018.g005