MicroRNA-Mediated Myostatin Silencing in Caprine Fetal Fibroblasts
Figure 5
Knockdown of myostatin and induction of the IFN response in CFF cells.
Each of the two pDG-miRNA plasmids was transfected into CFF cells transiently (A∼C) and stably (A, B, D). The mRNA levels of myostatin (A) and two IFN stimulated genes (IFN-β and OAS2) (B∼D) were determined by qPCR, which were normalized to GAPDH levels. PC: parental fetal myoblasts control; NC: pDG-miRNA-NC; m1: pDG-miRNA-1; m3: pDG-miRNA-3; TT: transient transfection; ST: stable transfection. *p<0.05.