A Protein L -Based Immunodiagnostic Approach Utilizing Time-Resolved Förster Resonance Energy Transfer
Figure 8
Protein L vs. FRET-bridge assay.
Dilutions series (25–3.1 nM) of the antibodies, A) anti-SA antibody and B) anti-GST, were tested in parallel with both of the assay approaches. Protein L was titrated along with the antibody concentration, representing concentrations from 50 to 6.3 nM. Concentrations of GST-VP1u and SA were constant at 10 nM. The third line in both figures represents a control for fluorescence background induced by AF-labeled protein L and the antigen. The y-axis represents response counts obtained from Victor2 fluorometer. The error bars represent ± standard deviation between parallel wells.