The Small GTP-Binding Proteins AgRho2 and AgRho5 Regulate Tip-Branching, Maintenance of the Growth Axis and Actin-Ring-Integrity in the Filamentous Fungus Ashbya gossypii
Figure 1
Growth of Agrho2 mutant strains.
A) Measurement of the radial growth speed on solid medium at 30° for the wild type, Agrho2 deletion and a strain carrying a GTP-locked allele of Agrho2. Shown are the arithmetic mean and standard deviation (n> = 3). The diameter of the initial inoculum was subtracted from each time-point, such that all measurements began at 0 mm. B) Example images of mycelia from the measurements in A) that display the difference in diameter taken at day 6 of the measurements. C) Measurement of the radial growth speed on solid medium at 30° for the wild type and Agrho2 overexpression. Shown are the arithmetic mean and standard deviation (n = 2). The diameter of the initial inoculum was subtracted from each time-point, such that all measurements began at 0 mm. D) Example images of mycelia from the measurements in C) that display the difference in diameter taken at day 6 of the measurements.