Hypertension and Hyperglycemia Synergize to Cause Incipient Renal Tubular Alterations Resulting in Increased NGAL Urinary Excretion in Rats
Figure 7
Increased NGAL urinary excretion is due to a tubular handling alteration.
A) Representative image of Western blot showing the effect of in situ perfusion of the renal vasculature with Krebs-dextran solution on NGAL urinary excretion in 3-month hyperglycemic SHR rats. B represents a basal urine sample before perfusing Krebs solution, when blood still perfuses the kidney. B) Representative image of Western blot showing the effect of adding an excess of NGAL to the Krebs-dextran solution on NGAL urinary excretion in 3-month normoglycemic (NG) and hyperglycemic SHR; and band quantification (lower graphic). C) Representative image of Western blot showing the effect of renal perfusion with the animal’s own blood from the carotid artery on NGAL urinary excretion in 3-month normoglycemic (NG) and hyperglycemic SHR; and band quantification (lower graphic). Data represent the average ± standard error of n = 5 per group. *p<0.001 vs. normoglycemic (NG) SHR. AU, arbitrary units.