The Co-Injection of Somatic Cells with Embryonic Stem Cells Affects Teratoma Formation and the Properties of Teratoma-Derived Stem Cell-Like Cells
Figure 4
Quantification of mRNA expression.
Five pluripotency-regulatory genes including BMP1, NGF, TGFB3, WNT5A, and WNT8A were selected from 839 probes showing both differential expression between parental ESCs and Group I and no differential expression between parental ESCs and Group II on the basis of microarray results. Three different ESC-like cells derived from the injection of heterologous ESCs alone, somatic cell co-injection with heterologous ESCs, and homologous ESCs alone were provided for quantitative RT-PCR analysis. For side by side comparison, the results were presented along with microarray results corresponding to each of genes. Similar gene expression pattern to microarray was detected in all five genes analyzed. Statistical analysis was performed by ANOVA-DUNCAN in SAS program. The p value in each graph indicates model effect among quantitative RT-PCR levels.