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Surface Survival and Internalization of Salmonella through Natural Cracks on Developing Cantaloupe Fruits, Alone or in the Presence of the Melon Wilt Pathogen Erwinia tracheiphila

Figure 5

Confocal laser scanning microscope images of cantaloupe rind surfaces.

(A) Fruit rind surface inoculated with S. enterica Poona (labeled with DsRedExpress) and sampled at 0 DPI, (B) Fruit rind surface inoculated with a mixture of S. enterica Poona + E. tracheiphila (labeled with GFPuv) and sampled at 0 DPI (C) Fruit rind surface inoculated with E. tracheiphila and sampled at 0 DPI and (D) Longitudinal section of rind containing watersoaked lesion and sampled at 24 DPI; E. tracheiphila in the intercellular spaces (arrow) (inoculated with mixture of S. enterica plus E. tracheiphila). The scale bars represent 5 µm.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0105248.g005