The Transient Receptor Potential Vanilloid 2 Cation Channel Is Abundant in Macrophages Accumulating at the Peri-Infarct Zone and May Enhance Their Migration Capacity towards Injured Cardiomyocytes following Myocardial Infarction
Figure 2
Elevated protein levels of TRPV2 and IGF-1 following acute MI.
(A.1) Surface levels of TRPV2 at various time points post MI compared to sham determined by flow cytometry analysis. Cells positive for TRPV2 expression are gated and the percentages are given (A.2). Total TRPV2 protein level three days post MI compared to sham analyzed by Western blot. Left panel of the membrane was blotted with TRPV2 antibody and the right panel was blotted with TRPV2 antibody pre-incubated with a TRPV2 peptide (1∶1 ratio). (B) IGF-1 expression level three days post MI, detected by Western blot analysis.